Rat MMP-9 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 1.56-100 ng/mL

Sandwich ELISA: 0.313-20 ng/mL

Cat No : MP00757-1

Print datasheet

Synonyms

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00757-1

  Sandwich ELISA standard curve of MP00757-1, Rat MMP-9 Recombinant Matched Antibody Pair - PBS only. 83768-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0439. 83768-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00757-1

  Serum of sixteen rats was measured. The rat MMP-9 concentration of detected samples was determined to be 25.36 ng/mL with a range of 19.61 - 33.20 ng/mL.

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  Cytometric bead array standard curve of MP00757-1

  Cytometric bead array standard curve of MP00757-1, RAT MMP-9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83768-2-PBS. Detection antibody: 83768-1-PBS. Standard: Eg0439. Range: 1.56-100 ng/mL.

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Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00757-1 targets MMP-9 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 1.56-100 ng/mL (Cytometric Bead Array); 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: Rat Mmp9 Recombinant antibody, PBS Only (Capture) 83768-2-PBS (240895B5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Rat Mmp9 Recombinant antibody, PBS Only (Detector) 83768-1-PBS (240895A8). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: Eg0439

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 83768-2-PBS
• Tested Reactivity: rat
• Immunogen: Fusion Protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: matrix metallopeptidase 9
• Synonyms: matrix metallopeptidase 9, 240895B5
• GenBank accession number: NM_031055.2
• Gene symbol: Mmp9
• Uniprot: P50282
• Gene ID (NCBI): 81687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 83768-1-PBS
• Tested Reactivity: rat
• Immunogen: Fusion Protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: matrix metallopeptidase 9
• Synonyms: matrix metallopeptidase 9, 240895A8
• GenBank accession number: NM_031055.2
• Gene symbol: Mmp9
• Uniprot: P50282
• Gene ID (NCBI): 81687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.