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Rat CXCL1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.3-2000 pg/mL

Cat No : MP00698-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00698-3, Rat Cxcl1 Recombinant Matched Antibody Pair - PBS only. 83745-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0948. 83745-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

    Sandwich ELISA standard curve of MP00698-3

    Sandwich ELISA standard curve of MP00698-3, Rat Cxcl1 Recombinant Matched Antibody Pair - PBS only. 83745-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0948. 83745-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

  • Serum of sixteen rats was measured. The rat Cxcl1 concentration of detected samples was determined to be 357.1 pg/mL with a range of 143.6 - 694.0 pg/mL.

    Sample test of MP00698-3

    Serum of sixteen rats was measured. The rat Cxcl1 concentration of detected samples was determined to be 357.1 pg/mL with a range of 143.6 - 694.0 pg/mL.

  • Rat lung (1 lung, 1-2 mm pieces) were cultured for 3 days in RPMI1640 plus 15% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 465.9 pg/mL; Rat spleen (1/2 spleen; 1-2 mm pieces) were cultured for 3 days in 50 mL DMEM supplemented with 10% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 17,149.5 pg/mL.

    Sample test of MP00698-3

    Rat lung (1 lung, 1-2 mm pieces) were cultured for 3 days in RPMI1640 plus 15% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 465.9 pg/mL; Rat spleen (1/2 spleen; 1-2 mm pieces) were cultured for 3 days in 50 mL DMEM supplemented with 10% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 17,149.5 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00698-3 targets CXCL1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.3-2000 pg/mL (Sandwich ELISA);

Capture antibody: Rat CXCL1 Recombinant antibody, PBS Only (Capture) 83745-4-PBS (240799G5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Rat CXCL1 Recombinant antibody, PBS Only (Detector) 83745-2-PBS (240799D7). 100 μg. Concentration 1 mgl/ml.

Alternative CXCL1 matched antibody pairs: MP00698-1, MP00698-2, MP00922-1

Matched pair protein standard: Eg0948

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83745-4-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Synonyms 240799G5
GenBank accession numberNM_030845.1
Gene symbol Cxcl1
UniprotP14095
Gene ID (NCBI) 81503
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83745-2-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Synonyms 240799D7
GenBank accession numberNM_030845.1
Gene symbol Cxcl1
UniprotP14095
Gene ID (NCBI) 81503
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.