RYBP Polyclonal antibody

RYBP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11365-1-AP


AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inmouse spleen tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11365-1-AP targets RYBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RYBP fusion protein Ag1908
Full Name RING1 and YY1 binding protein
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC014959
Gene Symbol RYBP
Gene ID (NCBI) 23429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]


Product Specific Protocols
WB protocol for RYBP antibody 11365-1-APDownload protocol
IP protocol for RYBP antibody 11365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan