RWDD1 Polyclonal antibody

RWDD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17036-1-AP

Synonyms

CGI 24, DFRP2, PTD013, RWD domain containing 1, RWDD1

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Validation Data Gallery

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  WB analysis using 17036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 17036-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 17036-1-AP

  IP result of anti-RWDD1 (IP:17036-1-AP, 4ug; Detection:17036-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of mouse kidney using 17036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17036-1-AP (RWDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

17036-1-AP targets RWDD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RWDD1 fusion protein Ag10525
• Full Name: RWD domain containing 1
• Calculated Molecular Weight: 243 aa, 28 kDa
• Observed Molecular Weight: 28-36 kDa
• GenBank Accession Number: BC015802
• Gene Symbol: RWDD1
• Gene ID (NCBI): 51389
• RRID: AB_2184704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RWDD1(RWD domain-containing protein 1) is also named as DFRP2(DRG family-regulatory protein 2) and belongs to the RWDD1/GIR2 family. RWDD1 is a thymus aging-related protein which contains an RWD domain at its N-terminus and it is expressed in both thymocytes and thymic epithelial cells and located primarily in the cytoplasm. This protein expression in thymus is decreased in aged and oxidatively stressed mice (PMID:18954556). RWDD1 has a calculated molecular mass of 28 kDa, and apparent molecular mass of 32-36 kDa due to its high acidic amino acid composition that results in the slowed migration behavior in electrophoresis (PMID: 30278355, 18954556).

Publications

Species	Application	Title
mouse	WB,IHC	Mol Cell Endocrinol; RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation.; Authors - Gr?tsch Helga H