RSAD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RSAD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 28089-1-AP

Synonyms

2510004L01Rik, cig33, cig5, RSAD2, vig1, Viperin

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Validation Data Gallery

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  WB analysis using 28089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28089-1-AP (RSAD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 28089-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28089-1-AP (RSAD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, A549 cells, mouse spinal cord tissue
• Positive IHC detected in: mouse stomach tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

28089-1-AP targets RSAD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSAD2 fusion protein Ag27733
• Full Name: radical S-adenosyl methionine domain containing 2
• Calculated Molecular Weight: 361 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC017969
• Gene Symbol: RSAD2
• Gene ID (NCBI): 91543
• RRID: AB_2881057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production; Authors - Sayuri Sakuragi
human	IHC	Am J Cancer Res; Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma; Authors - Yuanfeng Wei
human	WB	Cell Mol Biol Lett; 5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo; Authors - Sushan Yang
mouse	WB	Front Nutr; The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics.; Authors - Qiongxin Liang
mouse	WB	J Appl Toxicol; Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.; Authors - Xiaomin Tang
human	WB,IF,IP	Biochemistry; Interactions between Viperin, Vesicle-Associated Membrane Protein A, and Hepatitis C Virus Protein NS5A Modulate Viperin Activity and NS5A Degradation.; Authors - Soumi Ghosh