RRS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RRS1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15329-1-AP

Synonyms

KIAA0112, RRR, RRS1

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Validation Data Gallery

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  WB analysis of K-562 using 15329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15329-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15329-1-AP

  IP Result of anti-RRS1 (IP:15329-1-AP, 4ug; Detection:15329-1-AP 1:800) with K-562 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15329-1-AP targets RRS1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRS1 fusion protein Ag7030
• Full Name: RRS1 ribosome biogenesis regulator homolog (S. cerevisiae)
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 41 kDa
• GenBank accession number: BC001811
• Gene symbol: RRS1
• Gene ID (NCBI): 23212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan