RRS1 Polyclonal antibody

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  WB analysis of K-562 using 15329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15329-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15329-1-AP

  IP result of anti-RRS1 (IP:15329-1-AP, 4ug; Detection:15329-1-AP 1:800) with K-562 cells lysate 3200ug.

• Featured Product
• KD/KO Validated

RRS1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 15329-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

KIAA0112, RRR

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

15329-1-AP targets RRS1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7030

  Product name: Recombinant human RRS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-365 aa of BC001811

  Sequence: MEGQSVEELLAKAEQDEAEKLQRITVHKELELQFDLGNLLASDRNPPTGLRCAGPTPEAELQALARDNTQLLINQLWQLPTERVEEAIVARLPEPTTRLPREKPLPRPRPLTRWQQFARLKGIRPKKKTNLVWDEVSGQWRRRWGYQRARDDTKEWLIEVPGNADPLEDQFAKRIQAKKERVAKNELNRLRNLARAHKMQLPSAAGLHPTGHQSKEELGRAMQVAKVSTASVGRFQERLPKEKVPRGSGKKRKFQPLFGDFAAEKKNQLELLRVMNSKKPQLDVTRATNKQMREEDQEEAAKRRKMSQKGKRKGGRQGPGGKRKGGPPSQGGKRKGGLGGKMNSGPPGLGGKRKGGQRPGGKRRK

• Full Name: RRS1 ribosome biogenesis regulator homolog (S. cerevisiae)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC001811
• Gene Symbol: RRS1
• Gene ID (NCBI): 23212
• RRID: AB_2301118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15050
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Biol Direct; Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism; Authors - Akihiro Yamashita
human	WB	Tissue Cell; Silencing ribosome biogenesis regulator 1 homolog (RRS1) inhibits angiogenesis and cisplatin resistance of lung cancer cells by activating ferroptosis mediated by p53 pathway; Authors - Ling Lin
mouse	WB	Adv Sci (Weinh); m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control; Authors - Lisha Yin