RPP25 Polyclonal antibody

RPP25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15461-1-AP

Synonyms

RNase P protein subunit p25

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Validation Data Gallery

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  WB analysis of HeLa using 15461-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15461-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15461-1-AP

  IP result of anti-RPP25 (IP:15461-1-AP, 4ug; Detection:15461-1-AP 1:300) with HeLa cells lysate 2800 ug.

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  IHC staining of human cervical cancer using 15461-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 15461-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15461-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 15461-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 15461-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15461-1-AP (RPP25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human small intestine tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15461-1-AP targets RPP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPP25 fusion protein Ag7742
• Full Name: ribonuclease P/MRP 25kDa subunit
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC002497
• Gene Symbol: RPP25
• Gene ID (NCBI): 54913
• RRID: AB_2878142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA.

Publications

Species	Application	Title
human	WB	Mol Cell; Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.; Authors - Ok Hyun Park