RPN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPN2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10576-1-AP

Synonyms

RIBIIR, Ribophorin 2, ribophorin II, RPN II, RPN2, RPNII, SWP1

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 10576-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10576-1-AP (RPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 10576-1-AP

  IP result of anti-RPN2 (IP:10576-1-AP, 4ug; Detection:10576-1-AP 1:700) with NIH/3T3 cells lysate 3200ug.

• 

  IHC staining of human lung cancer using 10576-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10576-1-AP (RPN2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10576-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10576-1-AP (RPN2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10576-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10576-1-AP (RPN2 antibody) at dilution of 1:100 (under 25x lens).

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Product Information

10576-1-AP targets RPN2 in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPN2 fusion protein Ag0862
• Full Name: ribophorin II
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68-69 kDa
• GenBank Accession Number: BC003560
• Gene Symbol: RPN2
• Gene ID (NCBI): 6185
• RRID: AB_2172805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPN2 (Ribophorin II), an integral rough ER membrane glycoprotein, is part of an N-oligosaccharyl transferase complex and is involved in translocation and the maintenance of the structural uniqueness of the rough ER. RPN2 expression has been reported to be significantly associated with solid cancers, including breast cancer, esophageal squamous cell carcinoma, colorectal cancer, and lung squamous cell carcinoma.

Publications

Species	Application	Title
human	IHC	Onco Targets Ther; RPN2 Predicts Poor Prognosis and Promotes Bladder Cancer Growth and Metastasis via the PI3K-Akt Pathway.; Authors - Chenglin Han
human	WB,IHC	Onco Targets Ther; Downregulation of ribophorin II suppresses tumor growth, migration, and invasion of nasopharyngeal carcinoma.; Authors - Feilong Hong ; KD Validated
mouse	WB	Res Sq; Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction; Authors - Hossein Ardehali
human	RIP	Theranostics; The N6-methyladenosine-mediated lncRNA WEE2-AS1 promotes glioblastoma progression by stabilizing RPN2; Authors - Boyan Li