Ribophorin I Polyclonal antibody

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  WB analysis using 12894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12894-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Ribophorin I Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12894-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

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Tested Applications

• Positive WB detected in: HT-29 cells, K-562 cells, HeLa cells
• Positive IHC detected in: mouse lung tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below

Product Information

12894-1-AP targets Ribophorin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ribophorin I fusion protein Ag3576
• Full Name: ribophorin I
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 66-69 kDa
• GenBank Accession Number: BC010839
• Gene Symbol: RPN1
• Gene ID (NCBI): 6184
• RRID: AB_2170474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04843
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribophorin I, also named as RPN1, is a key protein component of the oligosaccharyltransferase (OST) complex, which is embedded in the membrane of the rough endoplasmic reticulum (RER) (PMID: 38373565). It plays a crucial role in the process of N-linked glycosylation, a post-translational modification essential for the proper folding, stability, and function of many secretory and membrane-bound proteins (PMID: 17264154). Ribophorin I, along with Ribophorin II, forms a heterodimeric complex that facilitates the transfer of oligosaccharides from a lipid-linked donor to specific asparagine residues on nascent polypeptide chains (PMID: 30940778).

Publications

Species	Application	Title
yeast	WB	Nature; Reconfiguration of the proteasome during chaperone-mediated assembly.; Authors - Soyeon Park
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang
human	WB	Proteomics; Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.; Authors - Yini Wang