|Positive WB detected in||HeLa cells, K-562 cells|
|Positive IHC detected in||mouse lung tissue, rat liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12894-1-AP targets RPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPN1 fusion protein Ag3576|
|Full Name||ribophorin I|
|Calculated molecular weight||69 kDa|
|Observed molecular weight||66-69 kDa|
|GenBank accession number||BC010839|
|Gene ID (NCBI)||6184|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
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