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Ribophorin I Polyclonal antibody

Ribophorin I Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12894-1-AP

Synonyms

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit



Tested Applications

Positive WB detected inHT-29 cells, K-562 cells, HeLa cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12894-1-AP targets Ribophorin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ribophorin I fusion protein Ag3576
Full Name ribophorin I
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 66-69 kDa
GenBank Accession NumberBC010839
Gene Symbol RPN1
Gene ID (NCBI) 6184
RRIDAB_2170474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Ribophorin I antibody 12894-1-APDownload protocol
IHC protocol for Ribophorin I antibody 12894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
yeastWB

Nature

Reconfiguration of the proteasome during chaperone-mediated assembly.

Authors - Soyeon Park
mouseWB

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
humanWB

Sci Adv

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Authors - Chatchai Phoomak
humanWB

Nat Chem Biol

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Authors - Cecilia Lopez-Sambrooks
ratWB

Int J Mol Sci

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Authors - Wenjuan Zhang
humanWB

Proteomics

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Authors - Yini Wang