RPL5 Polyclonal antibody

RPL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Monkey and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 29092-1-AP

Synonyms

60S ribosomal protein L5, DBA6, MSTP030, ribosomal protein L5, RPL5

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Validation Data Gallery

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  WB analysis using 29092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29092-1-AP (RPL5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 29092-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29092-1-AP (RPL5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 29092-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29092-1-AP (RPL5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COS-7 cells, MOLT-4 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

29092-1-AP targets RPL5 in WB, IHC, ELISA applications and shows reactivity with Human, Monkey samples.

• Tested Reactivity: Human, Monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL5 fusion protein Ag30837
• Full Name: ribosomal protein L5
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC109370
• Gene Symbol: RPL5
• Gene ID (NCBI): 6125
• RRID: AB_2881240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome is a macromolecular assembly of 4 RNA species (rRNAs) and approximately 80 different proteins, including RPL5. RPL5 (ribosome protein L15) is rquired for rRNA maturation and formation of the 60S ribosomal subunits, and defects or mutation in RPL5 is the cause of DBA6.

Publications

Species	Application	Title
human	WB	Cell Rep; mTOR- and LARP1-dependent regulation of TOP mRNA poly(A) tail and ribosome loading; Authors - Koichi Ogami
human	WB	BMC Mol Cell Biol; Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway; Authors - Huahua Zhang ; KD Validated
human	IHC	Front Oncol; Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer; Authors - Hong-Tao Liu
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi