RPL36 Polyclonal antibody

RPL36 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15145-1-AP

Synonyms

ribosomal protein L36, Large ribosomal subunit protein eL36, 60S ribosomal protein L36

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Validation Data Gallery

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  WB analysis of PC-12 using 15145-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15145-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15145-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15145-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15145-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15145-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15145-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL36 antibody (15145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: PC-12 cells, BxPC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15145-1-AP targets RPL36 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL36 fusion protein Ag7437
• Full Name: ribosomal protein L36
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12-14 kDa
• GenBank Accession Number: BC003052
• Gene Symbol: RPL36
• Gene ID (NCBI): 25873
• RRID: AB_2878111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL36, also named as 60S ribosomal protein L36, is a 105 amino acid protein, which is a component of the large ribosomal subunit. RPL36 may be involved in the early stage of hepatocarcinogenesis, and it can be used as an independent and potential prognostic marker for resected HCC.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; METTL3 preferentially enhances non-m6A translation of epigenetic factors and promotes tumourigenesis; Authors - Xueju Wei
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren