Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17619-1-AP targets RPL26 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, monkey, c.elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11821 Product name: Recombinant human RPL26 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC071664 Sequence: MKFNPFVTSDRSKNRKRHFNAPSHIRRKIMSSPLSKELRQKYNVRSMPIRKDDEVQVVRGHYKGQQIGKVVQVYRKKYVIYIERVQREKANGTTVHVGIHPSKVVITRLKLDKDRKKILERKAKSRQVGKEKGKYKEETIEKMQE Predict reactive species |
| Full Name | ribosomal protein L26 |
| Calculated Molecular Weight | 145 aa, 17 kDa |
| Observed Molecular Weight | 17-22 kDa |
| GenBank Accession Number | BC071664 |
| Gene Symbol | RPL26 |
| Gene ID (NCBI) | 6154 |
| RRID | AB_2878420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61254 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL26 antibody 17619-1-AP | Download protocol |
| WB protocol for RPL26 antibody 17619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RPL26 (Cat# 17619-1-AP) has 13 citations published in journals including Science, Nature Cell Biology, Nature Communications, Brain, BMC Genomics, American Journal of Cancer Research, Genes, Journal of Thermal Biology, Zoological Research, Biophysical Reports, and bioRxiv. Research fields span ribophagy, ER-mitochondrial crosstalk, breast and lung cancer progression, erythroid genome integrity, NOTCH2NLC-related neurodegeneration, TDP-43 pathology, muscle protein synthesis, and transcriptional regulation of CD40.
| Species | Application | Title |
|---|---|---|
Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function | ||
Nat Commun The UFL1-AKT positive feedback loop promotes breast cancer progression by enhancing lipid synthesis. | ||
Nat Commun DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death | ||
Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation |
Reviews
What customers say
The single review for this antibody is a 5-star rating from July 2020. The user performed a CRISPR knock-in of RPL26 and reported that the antibody easily detected the target by Western Blot. They used a 1:2000 dilution on HCT116 cells with lot 17153 and included an image confirming the result. Overall, the reviewer was satisfied with the antibody's performance for detecting their RPL26 knock-in construct.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (07-09-2020) | This antibody works well. I did crispr knock-in of RPL26 and it was easily detected by this antibody
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