RPL26 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL26 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 17619-1-AP

Synonyms

ribosomal protein L26, Large ribosomal subunit protein uL24, 60S ribosomal protein L26

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Validation Data Gallery

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  WB analysis of K-562 using 17619-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17619-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17619-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17619-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17619-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPL26 antibody (17619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

17619-1-AP targets RPL26 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL26 fusion protein Ag11821
• Full Name: ribosomal protein L26
• Calculated Molecular Weight: 145 aa, 17 kDa
• Observed Molecular Weight: 17-22 kDa
• GenBank Accession Number: BC071664
• Gene Symbol: RPL26
• Gene ID (NCBI): 6154
• RRID: AB_2878420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	Genes (Basel); Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.; Authors - Meijuan Zou ; KD Validated
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang
human	WB	Brain; GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation; Authors - Yu Fan
human	WB,IHC,CoIP	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong
human	WB	J Therm Biol; Pre-heating stress associated with acute oral leucine supplementation effects in rat gastrocnemius muscle: Implications for protein synthesis signaling pathways; Authors - Toshinori Yoshihara

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody works well. I did crispr knock-in of RPL26 and it was easily detected by this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPL26 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:17619-1-AP)