RPA4 Polyclonal antibody

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  WB analysis of HeLa using 18144-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 18144-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18144-1-AP

  IP result of anti-RPA4 (IP:18144-1-AP, 4ug; Detection:18144-1-AP 1:300) with HeLa cells lysate 3400ug.

RPA4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 18144-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

HSU24186, Replication factor A protein 4, replication protein A4, 34kDa, RF A protein 4, RP A p30, RPA4

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Tested Applications

• Positive WB detected in: HeLa cells, mouse spleen tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18144-1-AP targets RPA4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPA4 fusion protein Ag12800
• Full Name: replication protein A4, 34kDa
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC069791
• Gene Symbol: RPA4
• Gene ID (NCBI): 29935
• RRID: AB_2180970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13156
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPA4, also named as RP-A p30, is a 261 amino acid protein, which belongs to the replication factor A protein 2 family. RPA4 is preferentially expressed in placental and colon mucosa. RPA4 as part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. This protein, Rpa4, has 47% identity with Rpa2, the 34-kDa subunit of RPA. (PMID: 7760808)