RNF219 Polyclonal antibody

RNF219 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24988-1-AP

Synonyms

C13orf7, ring finger protein 219, RNF219

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Validation Data Gallery

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  WB analysis of HEK-293T using 24988-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24988-1-AP (RNF219 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 24988-1-AP

  IP result of anti-RNF219 (IP:24988-1-AP, 4ug; Detection:24988-1-AP 1:300) with A431 cells lysate 1200ug.

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  IP experiment of HEK-293 using 24988-1-AP

  IP result of anti-RNF219 (IP:24988-1-AP, 4ug; Detection:24988-1-AP 1:300) with HEK-293 cells lysate 920ug.

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  IF Staining of HEK-293 using 24988-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24988-1-AP (RNF219 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IP detected in: A431 cells, HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24988-1-AP is RNF219 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF219 fusion protein Ag21767
• Full Name: ring finger protein 219
• Calculated Molecular Weight: 726 aa, 81 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC136764
• Gene Symbol: RNF219
• Gene ID (NCBI): 79596
• RRID: AB_2879832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.