RIMKLB Polyclonal antibody

• Featured Product
• KD/KO Validated

RIMKLB Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 26111-1-AP

Synonyms

FAM80B, KIAA1238, RIMKLB

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Validation Data Gallery

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  WB analysis of mouse eye using 26111-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 26111-1-AP (RIMKLB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 26111-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26111-1-AP (RIMKLB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26111-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26111-1-AP (RIMKLB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 26111-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using RIMKLB antibody (26111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 26111-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using RIMKLB antibody (26111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 26111-1-AP is RIMKLB Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIMKLB fusion protein Ag23631
• Full Name: ribosomal modification protein rimK-like family member B
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-48 kDa
• GenBank Accession Number: BC033793
• Gene Symbol: RIMKLB
• Gene ID (NCBI): 57494
• RRID: AB_2880383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Res; Comprehensive metabolomics expands precision medicine for triple-negative breast cancer.; Authors - Yi Xiao ; KD Validated
	WB	J Inherit Metab Dis; NAAG synthetase deficiency has only low influence on pathogenesis in a Canavan disease mouse model; Authors - Ivonne Becker

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (02-24-2023)

The antibody worked well at the recommended concentration (1:1000). Strong band was detected at ~46 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Loucy/T-ALL cell line