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  • KD/KO Validated

RICTOR Polyclonal antibody

RICTOR Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27248-1-AP

Synonyms

AVO3 homolog, hAVO3, KIAA1999, mAVO3, RICTOR



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27248-1-AP targets RICTOR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RICTOR fusion protein Ag25649
Full Name rapamycin-insensitive companion of mTOR
Calculated molecular weight 192 kDa
Observed molecular weight 192 kDa
GenBank accession numberBC029608
Gene symbol RICTOR
Gene ID (NCBI) 253260
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RICTOR antibody 27248-1-APDownload protocol
IHC protocol for RICTOR antibody 27248-1-APDownload protocol
IF protocol for RICTOR antibody 27248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanWB

Aging (Albany NY)

Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts

Authors - Shi-Yu Wang
mouseWB,IF

Cell Rep

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways

Authors - Tatsuki Yasuda
mouseIF

J Cell Physiol

Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes

Authors - Rui Hua
humanWB

Cell Commun Signal

Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.

Authors - Norihiko Sasaki
human,mouseWB

Phytomedicine

Spiropachysine A suppresses hepatocellular carcinoma proliferation by inducing methuosis in vitro and in vivo.

Authors - Yuan Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-12-2019)

The antibody recognizes RICTOR which is around ~200kD. It is the top most band. The first two lanes were cell lysates and show that the antibody recognizes other proteins non-specifically. The last lane contained eluted proteins that are presumably proximal to the cell membrane. RICTOR shows up there cleanly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
RICTOR Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:27248-1-AP)