Validation Data Gallery
|Positive WB detected in||HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells|
|Positive IHC detected in||human lung cancer tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
27248-1-AP targets RICTOR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RICTOR fusion protein Ag25649|
|Full Name||rapamycin-insensitive companion of mTOR|
|Calculated molecular weight||192 kDa|
|Observed molecular weight||192 kDa|
|GenBank accession number||BC029608|
|Gene ID (NCBI)||253260|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
A glycolytic shift in Schwann cells supports injured axons.
Aging (Albany NY)
Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts
Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways
J Cell Physiol
Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes
Cell Commun Signal
Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.
Spiropachysine A suppresses hepatocellular carcinoma proliferation by inducing methuosis in vitro and in vivo.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-12-2019)
The antibody recognizes RICTOR which is around ~200kD. It is the top most band. The first two lanes were cell lysates and show that the antibody recognizes other proteins non-specifically. The last lane contained eluted proteins that are presumably proximal to the cell membrane. RICTOR shows up there cleanly.