RHOV Polyclonal antibody

• Featured Product
• KD/KO Validated

RHOV Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26620-1-AP

Synonyms

ARHV, CDC42 like GTPase 2, Chp, GTP binding protein like 2, Rho GTPase like protein ARHV, RHOV, WRCH 2, WRCH2

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Validation Data Gallery

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  WB analysis of rat lung using 26620-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26620-1-AP (RHOV Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 26620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

26620-1-AP targets RHOV in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOV fusion protein Ag24331
• Full Name: ras homolog gene family, member V
• Calculated Molecular Weight: 236 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC112945
• Gene Symbol: RHOV
• Gene ID (NCBI): 171177
• RRID: AB_2880576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.; Authors - Deyu Zhang
human	WB,IHC	Front Oncol; Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma.; Authors - Hongjin Chen ; KD Validated