MIRO2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MIRO2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (2)

Cat no : 11237-1-AP

Synonyms

ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2

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Validation Data Gallery

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  WB analysis using 11237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11237-1-AP (MIRO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11237-1-AP

  WB result of MIRO2 antibody (11237-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIRO2 transfected HepG2 cells.

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  WB analysis using 11237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11237-1-AP (MIRO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11237-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11237-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11237-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11237-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11237-1-AP

  IP result of anti-MIRO2 (IP:11237-1-AP, 4ug; Detection:11237-1-AP 1:4000) with L02 cells lysate 3080 ug.

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  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11237-1-AP (RHOT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11237-1-AP (RHOT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11237-1-AP (RHOT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11237-1-AP (RHOT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11237-1-AP (RHOT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11237-1-AP (RHOT2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, human kidney tissue, mouse heart tissue, L02 cells, HepG2 cells, human testis tissue, human heart tissue, K-562 cells, Hela cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human colon tissue, human kidney tissue, human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Product Information

11237-1-AP targets MIRO2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIRO2 fusion protein Ag1752
• Full Name: ras homolog gene family, member T2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC014942
• Gene Symbol: RHOT2
• Gene ID (NCBI): 89941
• RRID: AB_2179539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle. MIRO2 is highly phosphorylated at basal conditions, as evidenced by an immunoreactive band that electrophoretically migrates 7 kDa higher (~75 kDa) compared to its predicted molecular weight (68 kDa) (PMID: 28556983).

Publications

Species	Application	Title
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
		Neuron; Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.; Authors - Juliet Goldsmith
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
human	IF	EMBO Rep; Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes.; Authors - Fumika Koyano
human	WB, IP, IF	EMBO Rep; MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.; Authors - Fei Wang
human	WB, IF	Mol Ther Nucleic Acids; miR-351-5p/Miro2 axis contributes to hippocampal neural progenitor cell death via unbalanced mitochondrial fission.; Authors - Ha-Na Woo ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mary (Verified Customer) (09-09-2024)

Worked very well so far!!

• Applications: Immunofluorescence

FH

Tim (Verified Customer) (11-25-2021)

Tested on HeLa total cell lysate. Very good.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

MIRO2 Antibody Western Blot validation (1/1000 dilution) in HeLa (Cat no:11237-1-AP)