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  • KD/KO Validated

MIRO2 Polyclonal antibody

MIRO2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 11237-1-AP


ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2

Tested Applications

Positive WB detected inHEK-293T cells, human kidney tissue, mouse heart tissue, L02 cells, HepG2 cells, human testis tissue, human heart tissue, K-562 cells, Hela cells
Positive IP detected inL02 cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11237-1-AP targets MIRO2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIRO2 fusion protein Ag1752
Full Name ras homolog gene family, member T2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014942
Gene Symbol RHOT2
Gene ID (NCBI) 89941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle. MIRO2 is highly phosphorylated at basal conditions, as evidenced by an immunoreactive band that electrophoretically migrates 7 kDa higher (~75 kDa) compared to its predicted molecular weight (68 kDa) (PMID: 28556983).


Product Specific Protocols
WB protocol for MIRO2 antibody 11237-1-APDownload protocol
IHC protocol for MIRO2 antibody 11237-1-APDownload protocol
IP protocol for MIRO2 antibody 11237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
  • KD Validated


Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Authors - Juliet Goldsmith

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico


Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes.

Authors - Fumika Koyano
humanWB, IP, IF


MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.

Authors - Fei Wang
humanWB, IF

Mol Ther Nucleic Acids

miR-351-5p/Miro2 axis contributes to hippocampal neural progenitor cell death via unbalanced mitochondrial fission.

Authors - Ha-Na Woo
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tim (Verified Customer) (11-25-2021)

Tested on HeLa total cell lysate. Very good.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
MIRO2 Antibody Western Blot validation (1/1000 dilution) in HeLa (Cat no:11237-1-AP)