RHOBTB3 Polyclonal antibody

RHOBTB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13945-1-AP

Synonyms

KIAA0878, RHOBTB3

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Validation Data Gallery

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  WB analysis using 13945-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13945-1-AP (RHOBTB3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 13945-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat adrenal gland using 13945-1-AP

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 13945-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13945-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, K-562 cells
• Positive IHC detected in: mouse heart tissue, human placenta tissue, rat adrenal gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

13945-1-AP targets RHOBTB3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOBTB3 fusion protein Ag5025
• Full Name: Rho-related BTB domain containing 3
• Calculated Molecular Weight: 611 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC041337
• Gene Symbol: RHOBTB3
• Gene ID (NCBI): 22836
• RRID: AB_2877992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.; Authors - Jiaxin Chen
human	WB,IP	Cells; RhoBTB3 Functions as a Novel Regulator of Autophagy by Suppressing AMBRA1 Stability; Authors - Kyungho Kim