RHEB Polyclonal antibody

• Featured Product
• KD/KO Validated

RHEB Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 15924-1-AP

Synonyms

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

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Validation Data Gallery

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  WB analysis using 15924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 15924-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 15924-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 15924-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15924-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human pancreas cancer using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas cancer using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15924-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHEB antibody (15924-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, human brain tissue, HeLa cells, 3T3-L1 cells, fetal human brain tissue
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human pancreas cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

15924-1-AP targets RHEB in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHEB fusion protein Ag8747
• Full Name: Ras homolog enriched in brain
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC016155
• Gene Symbol: RHEB
• Gene ID (NCBI): 6009
• RRID: AB_2285084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Publications

Species	Application	Title
rat	WB	Cell Metab; Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.; Authors - Noriaki Shimizu
mouse	IHC	PLoS Biol; RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.; Authors - Martina Proietti Onori
mouse	IF	Metabolism; Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.; Authors - Yan Yang ; KO Validated
mouse	IF	iScience; Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9; Authors - Ting Wang
human		Front Cell Dev Biol; PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.; Authors - Qingqiong Luo
mouse	WB	Stem Cell Res Ther; Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.; Authors - Caixia Jin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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