RHBDD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RHBDD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20869-1-AP

Synonyms

HSD 50, RHBDD1, RHBDL4, rhomboid domain containing 1

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 20869-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20869-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20869-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20869-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20869-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 20869-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20869-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, mouse testis tissue, PC-3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

20869-1-AP targets RHBDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHBDD1 fusion protein Ag14932
• Full Name: rhomboid domain containing 1
• Calculated Molecular Weight: 315 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC027900
• Gene Symbol: RHBDD1
• Gene ID (NCBI): 84236
• RRID: AB_10733756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.; Authors - Koji Shibuya
human	WB	Environ Toxicol; Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway.; Authors - Jiong Jiang ; KD Validated
human	WB	Cell Death Dis; Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.; Authors - Xiaohui Zhang
human	WB	Genes Cells; Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation; Authors - Shinichiro Yamamoto ; KD Validated