RGS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RGS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10678-1-AP

Synonyms

G0S8, GOS8, GIG31, G0/G1 switch regulatory protein 8, Cell growth-inhibiting gene 31 protein

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Validation Data Gallery

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  WB analysis of fetal human brain using 10678-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10678-1-AP (RGS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10678-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10678-1-AP (RGS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 10678-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Product Information

10678-1-AP targets RGS2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS2 fusion protein Ag1066
• Full Name: regulator of G-protein signaling 2, 24kDa
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24-30 kDa
• GenBank Accession Number: BC007049
• Gene Symbol: RGS2
• Gene ID (NCBI): 5997
• RRID: AB_2284998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; RGS2-mediated translational control mediates cancer cell dormancy and tumor relapse.; Authors - Jaebeom Cho ; KD Validated
human	WB	Basic Res Cardiol; Novel insights into the mechanisms mediating the local antihypertrophic effects of cardiac atrial natriuretic peptide: role of cGMP-dependent protein kinase and RGS2.; Authors - Klaiber Michael M
human	WB,IF	J Cell Physiol; Novel role of Giα2 in cell migration: Downstream of PI3-kinase-AKT and Rac1 in prostate cancer cells.; Authors - Silvia Caggia ; KD Validated
human	WB	Respir Res; Upregulation of RGS2: a new mechanism for pirfenidone amelioration of pulmonary fibrosis.; Authors - Yan Xie
human	WB	Virology; Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection; Authors - Li Xia Yuan
human	WB	Toxicol Appl Pharmacol; RGS2 attenuates alveolar macrophage damage by inhibiting the Gq/11-Ca2+ pathway during cowshed PM2.5 exposure, and aberrant RGS2 expression is associated with TLR2/4 activation; Authors - Zhenhua Ma