RGS10 Polyclonal antibody

• Featured Product
• KD/KO Validated

RGS10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11163-1-AP

Synonyms

Regulator of G-protein signaling 10

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Validation Data Gallery

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  WB analysis of mouse thymus using 11163-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11163-1-AP (RGS10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11163-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11163-1-AP (RGS10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11163-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11163-1-AP (RGS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11163-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11163-1-AP (RGS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 11163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11163-1-AP (RGS10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, K-562 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

11163-1-AP targets RGS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS10 fusion protein Ag1644
• Full Name: regulator of G-protein signaling 10
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC009361
• Gene Symbol: RGS10
• Gene ID (NCBI): 6001
• RRID: AB_10596933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int Endod J; RGS10 negatively regulates apical periodontitis via TFEB-mediated autophagy in BABL/c mice model and in vitro; Authors - Jiaxin Li
mouse	WB	Oral Dis; Inhibition of Rgs10 aggravates periodontitis with collagen-induced arthritis via the NF-κB pathway.; Authors - Wei-Cheng Chan ; KD Validated
mouse	WB	J Endod; Inhibition of RGS10 Aggravates Periapical Periodontitis via Upregulation of the NF-κB Pathway; Authors - Wei Wei