ChromoTek rat anti RFP Monoclonal antibody (5F8)

Rat monoclonal [5F8] to Red Fluorescent Proteins (RFP)

Host/IsoType

Rat/IgG2a

Specificity

RFP and RFP derivates

Applications

IF, ELISA

Conjugate

Unconjugated

Clone No.

5F8

Publications

670

Reviews (3)

Cat no : 5f8

Synonyms

RFP, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  HeLa cells transiently transfected with MannosidaseII-tdTomato (Golgi). Primary antibody: [5F8] 1:1,000 Secondary antibody: goat anti-rat Alexa Fluor® 647 Fixation: 3.7 % PFA Scale bar: 10 µm

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Product Information

Rat monoclonal [5F8] to Red Fluorescent Proteins (RFP)

• Description: RFP rat monoclonal [5F8] for IF, ELISA
• Applications: IF, ELISA
• Specificity/Target: mOrange, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, tdTomato, mKate2, mPlum; For the complete list, please click here: Fluorescent protein specificity table antibodies
• Recommended Dilution: Immunofluorescence: 1:1,000
• Host/IsoType: Rat/IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Red fluorescent protein (RFP)
• UniProtKB: Q9U6Y8
• Clone No.: 5F8
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A or G purified
• RRID: AB_2336064
• Storage Buffer: PBS, 50% glycerol, preservative: 0.09% sodium azide.
• Storage Condition: Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.
• Background: Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.; The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). ; When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.; This antibody is a rat monoclonal antibody raised against RFP, reactive to a variety of RFP derivates, including mOrange, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, tdTomato, mKate2, and mPlum.

Publications

Application	Title
IF	Nature; Fast and sensitive GCaMP calcium indicators for imaging neural populations; Authors - Yan Zhang
IHC	Nature; Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.; Authors - Joon-Hyuk Lee
IF	Nature; Identification of proliferative and mature β-cells in the islets of Langerhans.; Authors - Erik Bader
IF	Nature; Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.; Authors - David Willnow
IF	Nature; Equalizing excitation-inhibition ratios across visual cortical neurons.; Authors - Mingshan Xue
WB	Nature; Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios.; Authors - Philipp S Hoppe

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anzy (Verified Customer) (08-13-2024)

Works well against mScarlet-i

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: hES-derived neural progenitors

FH

Prabha (Verified Customer) (09-11-2023)

The RFP antibody 5F8 is really excellent. Immunodetection shows specific detection without any background signal.

• Applications: Western Blot,Immunoprecipitation,Protein purification
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Plant protein extracts

FH

Ramzi (Verified Customer) (01-24-2023)

I have tested the anti 5f8 Rat and the secondary I used anti-GFP Rat 488. I used vibratome brain sections from creER2 tomato DsRed mice. I tested in 2 different ratio 1:200 and 1:1000 both worked very will and this is the images I took.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200, 1:1000
• Cell Tissue Type: Brain Tissue