RFK Polyclonal antibody

RFK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 15813-1-AP

Synonyms

Flavokinase, RFK, riboflavin kinase, RIFK

---

Validation Data Gallery

• 

  WB analysis of HeLa using 15813-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15813-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15813-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15813-1-AP (RFK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15813-1-AP targets RFK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFK fusion protein Ag8591
• Full Name: riboflavin kinase
• Calculated Molecular Weight: 162 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC007069
• Gene Symbol: RFK
• Gene ID (NCBI): 55312
• RRID: AB_2178612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.

Publications

Species	Application	Title
mouse	WB	Transl Psychiatry; Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.; Authors - Michail S Kukharsky
mouse	WB	Eur J Immunol; Riboflavin (vitamin B2 ) deficiency impairs Nox2 priming and defense against Listeria monocytogenes.; Authors - Schramm Michael M
mouse	WB	Adv Sci (Weinh); Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation; Authors - Mengran Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tatyana (Verified Customer) (01-21-2023)

Works well in mouse tissue for IHC and WB. Standard antigen retrieval procedure and DAB IHC detection were used in the mouse spinal cord (overnight incubation at 4C). The protein primarily neuronal expression, showing weak but clean and clear signal. WB was done with semi-dry transfer and 4% milk in TBST.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse CNS tissue

RFK Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Mouse CNS tissue (Cat no:15813-1-AP)