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REDD1 specific Polyclonal antibody
REDD1 specific Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 10638-1-AP
Validation Data Gallery
|Positive WB detected in
|A431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10638-1-AP targets REDD1 specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, rabbit, pig, squirrel, gerbil
|Host / Isotype
|Rabbit / IgG
|REDD1 specific fusion protein Ag0965
|DNA-damage-inducible transcript 4
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)
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REDD1 is essential for stress-induced synaptic loss and depressive behavior.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yuki (Verified Customer) (11-10-2022)
This product was used for IHC in human tissue.
Stephane (Verified Customer) (11-26-2020)
The antibody performs great on human cell extract. It detects specific bands with the REDD1 band resolving around 30-35 kD.
Jamal (Verified Customer) (09-17-2019)
This product was used for Western Blotting in mouse cortex tissue.
Bradley (Verified Customer) (08-19-2019)
I have used this antibody on numerous occasions with success. The antibody performs great on human cell extract, as evidenced by a single immunoreactive band. The antibody performs moderately well on mouse skeletal muscle extract as it detects many non-specific bands with the REDD1 band resolving around 32-35 kD (specificity determined using REDD1-/- samples). The antibody is capable of detecting large changes in REDD1 protein in mouse skeletal muscle (i.e. induction following dexamethasone treatment), but smaller changes are difficult to assess in this tissue. This antibody will detect smaller changes in human cell lines such as HEK 293 or Hela cells.