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REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10638-1-AP

Synonyms

DDIT4, Dig2, REDD 1, REDD1, REDD1 specific, RP11 442H21.1, RTP801



Tested Applications

Positive WB detected inA431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10638-1-AP targets REDD1 specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, rabbit, pig, squirrel, gerbil
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REDD1 specific fusion protein Ag0965
Full Name DNA-damage-inducible transcript 4
Calculated molecular weight 25 kDa
Observed molecular weight 32-35 kDa
GenBank accession numberBC007714
Gene symbol DDIT4
Gene ID (NCBI) 54541
RRIDAB_2245711
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

Protocols

Product Specific Protocols
WB protocol for REDD1 specific antibody 10638-1-APDownload protocol
IHC protocol for REDD1 specific antibody 10638-1-APDownload protocol
IP protocol for REDD1 specific antibody 10638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Plzf regulates germline progenitor self-renewal by opposing mTORC1.

Authors - Hobbs Robin M RM
humanWB

Cell

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Authors - Zandra E Walton
mouseWB

Cancer Cell

A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

Authors - Isabelle K Vila
ratWB

Nat Med

REDD1 is essential for stress-induced synaptic loss and depressive behavior.

Authors - Kristie T Ota
mousechIP

Nat Immunol

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.

Authors - Wilson Castro
mouseWB

Acta Neuropathol

MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease.

Authors - Samir R Nath

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuki (Verified Customer) (11-10-2022)

This product was used for IHC in human tissue.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: kidney
REDD1 specific Antibody Immunohistochemistry validation (1:50 dilution) in kidney (Cat no:10638-1-AP)
FH

Stephane (Verified Customer) (11-26-2020)

The antibody performs great on human cell extract. It detects specific bands with the REDD1 band resolving around 30-35 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
REDD1 specific Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:10638-1-AP)
FH

Jamal (Verified Customer) (09-17-2019)

This product was used for Western Blotting in mouse cortex tissue.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Brain Tissue
FH

Bradley (Verified Customer) (08-19-2019)

I have used this antibody on numerous occasions with success. The antibody performs great on human cell extract, as evidenced by a single immunoreactive band. The antibody performs moderately well on mouse skeletal muscle extract as it detects many non-specific bands with the REDD1 band resolving around 32-35 kD (specificity determined using REDD1-/- samples). The antibody is capable of detecting large changes in REDD1 protein in mouse skeletal muscle (i.e. induction following dexamethasone treatment), but smaller changes are difficult to assess in this tissue. This antibody will detect smaller changes in human cell lines such as HEK 293 or Hela cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500 for mouse skeletal muscle. 1:1000 for human cells
  • Cell Tissue Type: Mouse skeletal muscle. HEK293 cells