REC8 Polyclonal antibody

REC8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10793-1-AP

Synonyms

Cohesin Rec8p, HR21spB, REC8, REC8 homolog (yeast), REC8L1, Rec8p

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Validation Data Gallery

• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10793-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human testis tissue, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

10793-1-AP targets REC8 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REC8 fusion protein Ag1250
• Full Name: REC8 homolog (yeast)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC010887
• Gene Symbol: REC8
• Gene ID (NCBI): 9985
• RRID: AB_2178282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).

Publications

Species	Application	Title
human	WB	Clin Cancer Res; Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients.; Authors - Ivan V Litvinov
mouse	IF	Cell Cycle; Knockout of cyclin B1 in granulosa cells causes female subfertility.; Authors - Jinmei Cheng
		Cell Cycle; Elevated intracellular pH appears in aged oocytes and causes oocyte aneuploidy associated with the loss of cohesion in mice.; Authors - Jin-Mei Cheng
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
human	IF	Int J Mol Sci; "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.; Authors - Kristine Salmina
human	WB	Exp Cell Res; The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells.; Authors - Erenpreisa Jekaterina J