RBM17 Polyclonal antibody, PBS Only

RBM17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13918-1-PBS

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

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Validation Data Gallery

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  WB analysis using 13918-1-AP (same clone as 13918-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 13918-1-AP (same clone as 13918-1-PBS)

  IP result of anti-RBM17 (IP:13918-1-AP, 4ug; Detection:13918-1-AP 1:10000) with MCF-7 cells lysate 1000 ug. This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13918-1-AP (same clone as 13918-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13918-1-AP (RBM17 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13918-1-AP (same clone as 13918-1-PBS)

  Immunofluorescent analysis of Hela cells, using RBM17 antibody 13918-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

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Product Information

13918-1-PBS targets RBM17 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM17 fusion protein Ag4921
• Full Name: RNA binding motif protein 17
• Calculated Molecular Weight: 401 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC039322
• Gene Symbol: RBM17
• Gene ID (NCBI): 84991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.