RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18893-1-AP

Synonyms

SCAF8, RNA-binding motif protein 16, RNA binding protein 16, KIAA1116, CDC5L complex-associated protein 7

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Validation Data Gallery

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  WB analysis of HT-1080 using 18893-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18893-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18893-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 18893-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18893-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18893-1-AP

  IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug.

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  IHC staining of human testis using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 18893-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 18893-1-AP

  Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells, L02 cells, HT-1080 cells, THP-1 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18893-1-AP targets RBM16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM16 fusion protein Ag13499
• Full Name: RNA binding motif protein 16
• Calculated Molecular Weight: 1271 aa, 141 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC070071
• Gene Symbol: RBM16
• Gene ID (NCBI): 22828
• RRID: AB_2175908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )