RASSF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

RASSF4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19118-1-AP

Synonyms

Ras association domain-containing protein 4, AD037

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Validation Data Gallery

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  WB analysis using 19118-1-AP

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 19118-1-AP (RASSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 19118-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19118-1-AP (RASSF4 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 19118-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19118-1-AP (RASSF4 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, NCI-H1299 cells, U2OS cells, MG-63 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

19118-1-AP targets RASSF4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASSF4 fusion protein Ag5780
• Full Name: Ras association (RalGDS/AF-6) domain family member 4
• Calculated Molecular Weight: 321 aa, 37 kDa
• Observed Molecular Weight: 34-37 kDa
• GenBank Accession Number: BC032593
• Gene Symbol: RASSF4
• Gene ID (NCBI): 83937
• RRID: AB_10596921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASSF4, a member of the classical RASSF family of scaffold proteins, is associated with alveolar rhabdomyosarcoma (aRMS), an aggressive pediatric cancer of muscle histogenesis. In aRMS, RASSF4 is upregulated in human cell lines and tumors and is oncogenic, portending a worse clinical prognosis. RASSF4 has been reported to directly interact with and activate K‐Ras in a GTP‐dependent manner. Ectopic expression of RASSF4 induces 293T apoptosis in a Ras‐dependent manner and inhibits tumour cell growth.

Publications

Species	Application	Title
human	WB,IHC	J Cell Mol Med; RASSF4 inhibits cell proliferation and increases drug sensitivity in colorectal cancer through YAP/Bcl-2 pathway.; Authors - Yong Han ; KD Validated
human	IHC	Tumour Biol; RASSF4 is downregulated in nonsmall cell lung cancer and inhibits cancer cell proliferation and invasion.; Authors - Yong Han