CoraLite® Plus 488-conjugated RASEF Monoclonal antibody

RASEF Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C2B2

Cat no : CL488-67159

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF



Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67159 targets RASEF in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149
Full Name RAS and EF-hand domain containing
Calculated Molecular Weight 740 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RASEF antibody CL488-67159Download protocol
Standard Protocols
Click here to view our Standard Protocols