RASEF Monoclonal antibody

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C2B2

Cat no : 67159-1-Ig

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF



Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67159-1-Ig targets RASEF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149
Full Name RAS and EF-hand domain containing
Calculated Molecular Weight 740 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2882456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.

Protocols

Product Specific Protocols
WB protocol for RASEF antibody 67159-1-IgDownload protocol
IHC protocol for RASEF antibody 67159-1-IgDownload protocol
IF protocol for RASEF antibody 67159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols