RASEF Polyclonal antibody

RASEF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28162-1-AP

Synonyms

Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45

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Validation Data Gallery

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  WB analysis using 28162-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28162-1-AP (RASEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 28162-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 28162-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 28162-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 28162-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, PC-12 cells
• Positive IHC detected in: human stomach tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28162-1-AP targets RASEF in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASEF fusion protein Ag28049
• Full Name: RAS and EF-hand domain containing
• Calculated Molecular Weight: 740 aa, 83 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC023566
• Gene Symbol: RASEF
• Gene ID (NCBI): 158158
• RRID: AB_2881079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASEF, or RAS and EF-hand domain-containing protein, is a member of the Rab GTPase protein family. RASEF is a protein with significant implications in lung cancer, potentially serving as both a prognostic biomarker and a therapeutic target. Its role in cancer cell growth and its interaction with ERK1/2 signaling make it a promising candidate for further research and development of new molecular therapies in lung cancer treatment.