RAI2 Polyclonal antibody

RAI2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28217-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 28217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28217-1-AP (RAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, IMR-32 cells, SK-N-SH cells, TT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28217-1-AP targets RAI2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAI2 fusion protein Ag28253
• Full Name: retinoic acid induced 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC027937
• Gene Symbol: RAI2
• Gene ID (NCBI): 10742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoic acid-induced 2 (RAI2) was initially described as a retinoic acid-inducible gene, which has been assumed to be involved in development. RAI2 was initially related to cell differentiation and induced by retinoic acid and has been identified as an emerging tumor suppressor in breast cancer and colorectal cancer. RAI2 is frequently methylated in human CRC, and the expression of RAI2 is regulated by promoter region methylation (PMID: 29796120).