Ager/Rage Recombinant antibody

Ager/Rage Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240774F11

Cat no : 83742-5-RR

Synonyms

Ager, RAGE, RAGE1, RAGE 1, MOK

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Validation Data Gallery

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  WB analysis using 83742-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 83742-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83742-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

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Tested Applications

• Positive WB detected in: rat lung tissue, mouse lung tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83742-5-RR is Ager/Rage Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: advanced glycosylation end product-specific receptor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42-55 kDa
• GenBank Accession Number: NM_007425.3
• Gene Symbol: Ager
• Gene ID (NCBI): 11596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).