RAB5A-Specific Polyclonal antibody

RAB5A-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20228-1-AP

Synonyms

RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A

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Validation Data Gallery

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  WB analysis of human brain using 20228-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20228-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20228-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20228-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20228-1-AP

  IP result of anti-RAB5A-Specific (IP:20228-1-AP, 4ug; Detection:20228-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 20228-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse colon tissue, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

20228-1-AP targets RAB5A-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB5A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_004162
• Gene Symbol: RAB5A
• Gene ID (NCBI): 5868
• RRID: AB_10694147
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

Publications

Species	Application	Title
mouse	IHC	Front Physiol; Overexpression of ameloblastin in secretory ameloblasts results in demarcated, hypomineralized opacities in enamel; Authors - Yong-Hee Patricia Chun