RAB3IP Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAB3IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12321-1-PBS

Synonyms

RAB3IP/Rabin8, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

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Validation Data Gallery

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  WB analysis of human brain using 12321-1-AP (same clone as 12321-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12321-1-AP (same clone as 12321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12321-1-AP (same clone as 12321-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 12321-1-AP (same clone as 12321-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 12321-1-AP (same clone as 12321-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 12321-1-AP (same clone as 12321-1-PBS)

  IP result of anti-RAB3IP (IP:12321-1-AP, 3ug; Detection:12321-1-AP 1:1000) with mouse lung tissue lysate 4500ug. This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12321-1-AP (same clone as 12321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12321-1-AP (same clone as 12321-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB3IP/Rabin8 antibody (12321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12321-1-PBS in a different storage buffer formulation.

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Product Information

12321-1-PBS targets RAB3IP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3IP fusion protein Ag2974
• Full Name: RAB3A interacting protein (rabin3)
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 53/51/43 kDa
• GenBank Accession Number: BC015548
• Gene Symbol: RAB3IP
• Gene ID (NCBI): 117177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.