RAB3GAP2 Monoclonal antibody

RAB3GAP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A4A1

Cat no : 66648-1-Ig

Synonyms

DKFZp434D245, FLJ14579, KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

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Validation Data Gallery

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  WB analysis using 66648-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66648-1-Ig (RAB3GAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66648-1-Ig targets RAB3GAP2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB3GAP2 fusion protein Ag20254
• Full Name: RAB3 GTPase activating protein subunit 2 (non-catalytic)
• Calculated Molecular Weight: 1393 aa, 156 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC146760
• Gene Symbol: RAB3GAP2
• Gene ID (NCBI): 25782
• RRID: AB_2882005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.