RAB27B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB27B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (1)

Cat no : 13412-1-AP

Synonyms

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2

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Validation Data Gallery

• 

  WB analysis of rat spleen using 13412-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13412-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 13412-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13412-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13412-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat stomach using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat stomach using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13412-1-AP (RAB27B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-87 MG using 13412-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB27B antibody (13412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: rat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: rat stomach tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human oesophagus cancer tissue, human pancreas cancer tissue, human prostate cancer tissue, human testis tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-87 MG cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 34 publications below
• IHC: See 13 publications below
• IF: See 9 publications below

Product Information

13412-1-AP targets RAB27B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB27B fusion protein Ag4064
• Full Name: RAB27B, member RAS oncogene family
• Calculated Molecular Weight: 218 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC027474
• Gene Symbol: RAB27B
• Gene ID (NCBI): 5874
• RRID: AB_2176732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Publications

Species	Application	Title
mouse	IHC	Nature; Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.; Authors - Lin Zhang
human	WB, IHC	Cell; Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.; Authors - Mirjam C Boelens
human	WB	Immunity; MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.; Authors - Seongmin Yoon
human	WB	Adv Sci (Weinh); Exosome Release Is Regulated by mTORC1.; Authors - Wenchong Zou
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kacie (Verified Customer) (09-12-2022)

Antibody works well for both ICC and WB.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: M17 Cells

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