RAB27A Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAB27A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17817-1-PBS

Synonyms

Rab-27, RAB27, Rab 27, GTP-binding protein Ram, GTP binding protein Ram

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Validation Data Gallery

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  WB analysis using 17817-1-AP (same clone as 17817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  WB analysis of K562 using 17817-1-AP (same clone as 17817-1-PBS)

  WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 17817-1-AP (same clone as 17817-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 17817-1-AP (same clone as 17817-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 17817-1-AP (same clone as 17817-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17817-1-AP (same clone as 17817-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 17817-1-AP (same clone as 17817-1-PBS)

  IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 17817-1-AP (same clone as 17817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 17817-1-AP (same clone as 17817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17817-1-PBS in a different storage buffer formulation.

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Product Information

17817-1-PBS targets RAB27A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB27A fusion protein Ag12136
• Full Name: RAB27A, member RAS oncogene family
• Calculated Molecular Weight: 221 aa, 25 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC107680
• Gene Symbol: RAB27A
• Gene ID (NCBI): 5873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.