RAB13 Polyclonal antibody

RAB13 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11718-1-AP

Synonyms

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

---

Validation Data Gallery

• 

  WB analysis using 11718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 11718-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (11718-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of K-562 using 11718-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug RAB13 Polyclonal antibody (11718-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11718-1-AP targets RAB13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB13 fusion protein Ag2292
• Full Name: RAB13, member RAS oncogene family
• Calculated Molecular Weight: 203 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000799
• Gene Symbol: RAB13
• Gene ID (NCBI): 5872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).