ProSAPiP1 Polyclonal antibody

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• KD/KO Validated

ProSAPiP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24936-1-AP

Synonyms

KIAA0552, ProSAP interacting protein 1, ProSAPiP1, ProSAPiP1 protein

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Validation Data Gallery

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  WB analysis using 24936-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 24936-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 24936-1-AP is ProSAPiP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ProSAPiP1 fusion protein Ag20765
• Full Name: ProSAPiP1 protein
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC038860
• Gene Symbol: ProSAPiP1
• Gene ID (NCBI): 9762
• RRID: AB_2714022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP	Neuron; Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.; Authors - Sophie Laguesse ; KD Validated