Phospho-TDP43 (Ser409/410) Monoclonal antibody, PBS Only

Phospho-TDP43 (Ser409/410) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2C1

Cat no : 66318-1-PBS

Synonyms

ALS10, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

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Validation Data Gallery

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  WB analysis of HeLa using 66318-1-Ig (same clone as 66318-1-PBS)

  Untreated and EA treated HeLa cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) Antibody) at dilution of 1:850 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66318-1-Ig (same clone as 66318-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66318-1-Ig (same clone as 66318-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 66318-1-Ig (same clone as 66318-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410)antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66318-1-Ig (same clone as 66318-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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Product Information

66318-1-PBS targets Phospho-TDP43 (Ser409/410) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa, 35 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.