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Phospho-TDP43 (Ser409/410) Polyclonal antibody, PBS Only

Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22309-1-PBS

Synonyms

TDP 43, TARDBP, TDP43, TDP-43, TAR DNA-binding protein 43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TDP43 (Ser409/410) Polyclonal antibody (22309-1-AP)

    WB analysis using 22309-1-AP (same clone as 22309-1-PBS)

    Non-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 22309-1-PBS in a different storage buffer formulation.

Product Information

22309-1-PBS targets Phospho-TDP43 (Ser409/410) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-50 kDa, 25-35 kDa
GenBank Accession NumberNM_007375
Gene Symbol TDP-43
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.