Phospho-TAU (Ser404) Recombinant antibody

Phospho-TAU (Ser404) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

5I16

Cat no : 81383-1-RR

Synonyms

DDPAC, FLJ31424, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, Phospho-TAU (Ser404), PPND, pSer404 Tau, TAU

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Validation Data Gallery

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  WB analysis of mouse brain using 81383-1-RR

  Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 81383-1-RR (Phospho-TAU (Ser404) antibody) at dilution of 1:16000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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Tested Applications

• Positive WB detected in: mouse brain tissue, λ phosphatase treated mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

81383-1-RR targets Phospho-TAU (Ser404) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

Publications

Species	Application	Title
mouse	WB	Neuromolecular Med; Lycium Barbarum Polysaccharides Improves Cognitive Functions in ICV-STZ-Induced Alzheimer's Disease Mice Model by Improving the Synaptic Structural Plasticity and Regulating IRS1/PI3K/AKT Signaling Pathway; Authors - Yingxi He
human,mouse	WB	J Med Chem; Discovery of Effective Dual PROTAC Degraders for Neurodegenerative Disease-Associated Aggregates; Authors - Wentao Zhu