Phospho-RPS6 (Ser236) Recombinant antibody

Phospho-RPS6 (Ser236) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB

Conjugate

Unconjugated

CloneNo.

7K17

Cat no : 80206-1-RR

Synonyms

40S ribosomal protein S6, Phosphoprotein NP33, Phospho-RPS6 (Ser236), ribosomal protein S6, Ribosome Marker, RPS6



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, Calyculin A treated HEK-293 cells, IGF-1 treated MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

80206-1-RR targets Phospho-RPS6 (Ser236) in WB applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 80206-1-RR specifically recognizes the phosphorylation site of Ser236 or dual site Ser235/236.

Protocols

Product Specific Protocols
WB protocol for Phospho-RPS6 (Ser236) antibody 80206-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols