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Phospho-PRKD1 (Ser916) Recombinant antibody, PBS Only

Phospho-PRKD1 (Ser916) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241786A11

Cat no : 80080-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PRKD1 (Ser916) Recombinant antibody (80080-2-RR)

    WB analysis using 80080-2-RR (same clone as 80080-2-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80080-2-RR (Phospho-PRKD1 (Ser916) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80080-2-PBS in a different storage buffer formulation.

Product Information

80080-2-PBS targets Phospho-PRKD1 (Ser916) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name protein kinase D1
Calculated Molecular Weight 102 kDa
Observed Molecular Weight115 kDa
GenBank Accession NumberNM_001330069
Gene Symbol PRKD1
Gene ID (NCBI) 5587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)