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Phospho-POLR2A (Ser7) Recombinant antibody, PBS Only

Phospho-POLR2A (Ser7) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240829F12

Cat no : 83932-1-PBS

Synonyms

POLR2A, EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-POLR2A (Ser7) Recombinant antibody (83932-1-RR)

    WB analysis using 83932-1-RR (same clone as 83932-1-PBS)

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 83932-1-RR (POLR2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83932-1-PBS in a different storage buffer formulation.

Product Information

83932-1-PBS targets Phospho-POLR2A (Ser7) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Calculated Molecular Weight 217 kDa
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_000937
Gene Symbol POLR2A
Gene ID (NCBI) 5430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.