Phospho-P62,SQSTM1 (Ser349) Polyclonal antibody

Phospho-P62,SQSTM1 (Ser349) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 29503-1-AP

Synonyms

P62, Phospho-p62/SQSTM1 (Ser349), sequestosome 1, SQSTM1, Ubiquitin binding protein p62

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Validation Data Gallery

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  WB analysis using 29503-1-AP

  Non-treated HeLa cells and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62,SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 29503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62,SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MG132 treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

29503-1-AP targets Phospho-P62,SQSTM1 (Ser349) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sequestosome 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC017222
• Gene Symbol: P62/SQSTM1
• Gene ID (NCBI): 8878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

Publications

Species	Application	Title
human,mouse	WB	Cell Death Differ; SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress; Authors - Jun Luo
mouse	WB	Chin Med; NBR1-p62-Nrf2 mediates the anti-pulmonary fibrosis effects of protodioscin; Authors - Qian Zeng
rat	WB	J Med Chem; A Lysosome-Targeting hNEU1 Inhibitor Treats Myocardial Infarction: A Potential Therapeutic Breakthrough; Authors - Wen Zhou
human	WB	J Biol Chem; Channel HCN4 mutation R666Q associated with sporadic arrhythmia decreases channel electrophysiological function and increases protein degradation; Authors - Hongrui Wang
human	WB	J Virol; The Porcine Cyclic GMP-AMP Synthase-STING Pathway Exerts an Unusual Antiviral Function Independent of Interferon and Autophagy; Authors - Sen Jiang
human	WB	Drug Des Devel Ther; α-Mangostin Inhibited M1 Polarization of Macrophages/Monocytes in Antigen-Induced Arthritis Mice by Up-Regulating Silent Information Regulator 1 and Peroxisome Proliferators-Activated Receptor γ Simultaneously; Authors - Yi-Jin Wu