Phospho-P62,SQSTM1 (Ser349) Polyclonal antibody

Phospho-P62,SQSTM1 (Ser349) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29503-1-AP

Synonyms

P62, Phospho-p62/SQSTM1 (Ser349), sequestosome 1, SQSTM1, Ubiquitin binding protein p62



Tested Applications

Positive WB detected inMG132 treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

29503-1-AP targets Phospho-P62,SQSTM1 (Ser349) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sequestosome 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

Protocols

Product Specific Protocols
WB protocol for Phospho-P62,SQSTM1 (Ser349) antibody 29503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Death Differ

SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress

Authors - Jun Luo
mouseWB

Chin Med

NBR1-p62-Nrf2 mediates the anti-pulmonary fibrosis effects of protodioscin

Authors - Qian Zeng
humanWB

J Biol Chem

Channel HCN4 mutation R666Q associated with sporadic arrhythmia decreases channel electrophysiological function and increases protein degradation

Authors - Hongrui Wang
humanWB

J Virol

The Porcine Cyclic GMP-AMP Synthase-STING Pathway Exerts an Unusual Antiviral Function Independent of Interferon and Autophagy

Authors - Sen Jiang
humanWB

Drug Des Devel Ther

α-Mangostin Inhibited M1 Polarization of Macrophages/Monocytes in Antigen-Induced Arthritis Mice by Up-Regulating Silent Information Regulator 1 and Peroxisome Proliferators-Activated Receptor γ Simultaneously

Authors - Yi-Jin Wu