Phospho-P62/SQSTM1 (Ser349) Polyclonal antibody

• 

  WB analysis using 29503-1-AP

  Non-treated HeLa cells and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 29503-1-AP

  Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Phospho-P62/SQSTM1 (Ser349) Polyclonal Antibody for WB, ELISA

Cat No. 29503-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

p p62 (Ser349), p p62 Ser349, p P62,SQSTM1, p P62,SQSTM1 (Ser349), p P62,SQSTM1 Ser349

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Tested Applications

• Positive WB detected in: MG132 treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

29503-1-AP targets Phospho-P62/SQSTM1 (Ser349) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sequestosome 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC017222
• Gene Symbol: P62/SQSTM1
• Gene ID (NCBI): 8878
• RRID: AB_2923592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13501
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Cell Death Differ; SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress; Authors - Jun Luo
mouse	WB	Chin Med; NBR1-p62-Nrf2 mediates the anti-pulmonary fibrosis effects of protodioscin; Authors - Qian Zeng
rat	WB	J Med Chem; A Lysosome-Targeting hNEU1 Inhibitor Treats Myocardial Infarction: A Potential Therapeutic Breakthrough; Authors - Wen Zhou
mouse	IF	Cell Death Dis; TMEM232 is required for the formation of sperm flagellum and male fertility in mice; Authors - Xinying Cai
human	WB	J Biol Chem; Channel HCN4 mutation R666Q associated with sporadic arrhythmia decreases channel electrophysiological function and increases protein degradation; Authors - Hongrui Wang
human	WB	J Virol; The Porcine Cyclic GMP-AMP Synthase-STING Pathway Exerts an Unusual Antiviral Function Independent of Interferon and Autophagy; Authors - Sen Jiang