Phospho-P53 (Ser392) Polyclonal antibody

Phospho-P53 (Ser392) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (1)





Cat no : 28963-1-AP


Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser392), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Tested Applications

Positive WB detected inλ phosphatase treated HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28963-1-AP targets Phospho-P53 (Ser392) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tumor protein p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC003596
Gene Symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.(PMID: 19935675, PMID: 24379683)


Product Specific Protocols
WB protocol for Phospho-P53 (Ser392) antibody 28963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Chem Biol Interact

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65

Authors - Jingwen Tan


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Andria (Verified Customer) (01-29-2023)

This antibody detects 2 bands. I believe the correct one is the lower because it is induced upon actinomycin treatment

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: NIH 3T3
Phospho-P53 (Ser392) Antibody Western Blot validation (1:500 dilution) in NIH 3T3 (Cat no:28963-1-AP)