Phospho-P53 (Ser392) Polyclonal antibody

Phospho-P53 (Ser392) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 28963-1-AP

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser392), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Validation Data Gallery

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  WB analysis using 28963-1-AP

  Non-treated HEK-293, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 28963-1-AP (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: λ phosphatase treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28963-1-AP targets Phospho-P53 (Ser392) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2918224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.(PMID: 19935675, PMID: 24379683)

Publications

Species	Application	Title
human	WB	Chem Biol Interact; Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65; Authors - Jingwen Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andria (Verified Customer) (01-29-2023)

This antibody detects 2 bands. I believe the correct one is the lower because it is induced upon actinomycin treatment

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NIH 3T3

Phospho-P53 (Ser392) Antibody Western Blot validation (1:500 dilution) in NIH 3T3 (Cat no:28963-1-AP)